Biopolymers. 2026 Jan;117(1):e70074. doi: 10.1002/bip.70074.
ABSTRACT
The effect of the enzyme-mediated poly(gallic acid) (PGAL) as a potential redox regulator or redox activity compound (RAC) on the morphology of human neuroblastoma SH-SY5Y cells and its methionine synthase (MS) activities is contrasted to those for disrupting compounds (EDC). For that, we study the effects of di(2-ethylhexyl)phthalate (DEHP) and monobutylphthalate (MBP) as common EDCs. The results show the expected significant decrease in cell density and predominance of phenotype N associated with shorter neurites after exposure to EDCs; however, homogeneous cell density and an S phenotype consistent with the control are observed after exposure to the polymeric RAC, and compared to other reported RAC metabolites, sulforaphane (SFN) and its precursor gallic acid (GA). Regarding the enzymatic activity of MS, a 64% increase is observed in the presence of EDCs. Surprisingly, control GA also shows a 35% increase in MS enzymatic activity, but this stable multiradical polyanion derivative has an average decrease of 51%. To the best of our knowledge, this is the first time that MS enzymatic activities-to-risk of endocrine disruption relationships, compared to that of a polymeric RAC, have been established using neuroblastoma cell cultures, laying groundwork for future research in neurobiology and environmental health.
PMID:41378671 | PMC:PMC12696780 | DOI:10.1002/bip.70074